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hek blue il 2 reporter system  (InvivoGen)


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    InvivoGen hek blue il 2 reporter system
    Hek Blue Il 2 Reporter System, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+cells/HEK-Blue+IL-2+Cells/10__1158_slash_2326___6066__cir___26___0459-101-9-13
    Average 95 stars, based on 38 article reviews
    hek blue il 2 reporter system - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Development of Bispecific Antibody Targeting Human IL-17A and IL-6
    Article Snippet: The binding data were analyzed with the ForteBio data analysis software HT 10.0, and affinity constants (KD) were calculated using a 1:1 interaction model (fitting global). .. HEK-Blue IL-6 (#hkb-il6; InvivoGen, San Diego, CA, USA) or HEK-Blue IL-17 (#hkb-il17; InvivoGen) reporter cells were used to assess the biological activity of VHHs. .. Cells were stimulated with IL-6 (1 ng/mL; PeproTech Inc., Rocky Hill, NJ, USA) and IL-17A (10 ng/mL; ACROBiosystems, Newark, DE, USA) in the presence of various concentrations of each VHH, ranging from 0.3 nM to 600 nM for IL-6 and from 0.003 nM to 100 nM for IL-17A.

    Cell Culture:

    Article Title: Nucleic acid nanostructure platform for programming immune stimulation
    Article Snippet: A fluorescent calibration curve was produced using serial dilutions of the free CpG overhang hybridized to the Cy5-conjugated ssDNA oligo and measured with the Tecan Spark to produce a regression line with which the coverage of CpGs on each NANP variant was determined. .. Reporter Cell Assays HEK-Blue TLR9 reporter cells and THP1-Dual reporter cells were purchased from Invivogen and cultured according to manufacturer protocols. .. One day prior to cell assays, TLR9 cells were detached from the flask, centrifuged at 250×g for 5 minutes at room temperature and resuspended in fresh growth medium, consisting of Dulbecco's Modified Eagle Medium with 4.5 g/L glucose (Sigma-Aldrich, cat. Code D6546-6×500ML), 10% heat-inactivated FBS (Fisher Scientific, cat. Code SH3007002HI), 100 U/mL penicillin, 100 ug/mL streptomycin (Life Technologies, cat. Code 15140148), 100 ug/mL Normocin (manufacturer, cat. Code) and 2 mM L-glutamine (Fisher Scientific, cat. Code SH3003401).

    Article Title: SdiA as a Repressor of Phagocytosis and Intracellular Survival in Klebsiella pneumoniae : Insights from Macrophage and Amoeba Models
    Article Snippet: .. THP1-Dual reporter cells (InvivoGen, cat. no. thpd-nfis) were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco 21875) supplemented with 10 % heat-inactivated fetal calf serum, 10 mM HEPES (Sigma), 100 U/ml penicillin, and 0.1 mg/ml streptomycin (Gibco). .. At 300-min time-point of macrophage infection assays (section 3.3 ), supernatant was recovered for mCXCL10, mTNFα, and mIL-1β quantification (Preprotech ELISA development Kit, ref.: 900-K153, 900-K54, and 900-K47 for each cytokine, respectively) using TMB Solution I (Biopanda, ref.: TMB-S-001) for colorimetric development.

    Activation Assay:

    Article Title: UC-associated autoantibodies to αvβ6 inhibit mucosal TGFβ activation and predispose to intestinal inflammation
    Article Snippet: .. TGFβ activation was assessed using HEK-Blue reporter cells (InvivoGen) plated on LAP-coated wells. .. Reporter activity was measured by SEAP production. αvβ6-expressing reporter cells were generated by transfecting HEK-Blue cells with β6 pcDNA1neo (Addgene plasmid #13580) using Lipofectamine 3000.

    Article Title: Lipidomic Analysis of Plasma Extracellular Vesicles from Adiponectin Deficient Mice or Metabolic Syndrome Patients Reveals Pro-Oxidative and Pro-Inflammatory Lipid Signatures Correlating with Metabolic Dysfunction.
    Article Snippet: Mitochondrial oxidative stress, particularly oxidation by superoxide, was measured by MitoSox Red (Thermo Fisher, M36008). .. RAW-dual reporter cells (InvivoGen, rawd-ismip) were seeded in 96-well plates and grown to 60%–70% confluency, then treated with EV for 4–24 h. The absorbance and luminescence, which indicates the activation of NF- κB dependent pathway and IRF3-dependent pathway, respectively, were quantified using QUANTI-Blue Solution and QUANTI-Luc 4 Lucia/Gaussia following manufacturer’s protocol. .. RNA in tissues and cells was extracted with Trizol (Invitrogen.

    Incubation:

    Article Title: CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2
    Article Snippet: .. After 24 h of incubation, 20 μL of supernatants from the reporter cells (HEK-Blue IFN-α/β or HEK-Blue IFN-λ) were added to 180 μL of Quanti-blue substrate (InvivoGen) and incubated at 37 °C for 1–2 h. Absorbance was measured at 650 nm using a microplate reader (PerkinElmer, EnVisionTM Multilabel Plate Reader). .. After 24 h of incubation, 20 μL of supernatants from the reporter cells (HEK-Blue IFN-α/β or HEK-Blue IFN-λ) were added to 180 μL of Quanti-blue substrate (InvivoGen) and incubated at 37 °C for 1–2 h. Absorbance was measured at 650 nm using a microplate reader (PerkinElmer, EnVision Multilabel Plate Reader).

    Article Title: CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2.
    Article Snippet: .. After 24 h of incubation, 20 μL of supernatants from the reporter cells (HEK-Blue IFN-α/β or HEK-Blue IFN-λ) were added to 180 μL of Quanti-blue substrate (InvivoGen) and incubated at 37 °C for 1–2 h. Absorbance was measured at 650 nm using a microplate reader (PerkinElmer, EnVisionTM Multilabel Plate Reader). ..

    96 Well Plates:

    Article Title: Lipidomic Analysis of Plasma Extracellular Vesicles from Adiponectin Deficient Mice or Metabolic Syndrome Patients Reveals Pro-Oxidative and Pro-Inflammatory Lipid Signatures Correlating with Metabolic Dysfunction.
    Article Snippet: Mitochondrial oxidative stress, particularly oxidation by superoxide, was measured by MitoSox Red (Thermo Fisher, M36008). .. RAW-dual reporter cells (InvivoGen, rawd-ismip) were seeded in 96-well plates and grown to 60%–70% confluency, then treated with EV for 4–24 h. The absorbance and luminescence, which indicates the activation of NF- κB dependent pathway and IRF3-dependent pathway, respectively, were quantified using QUANTI-Blue Solution and QUANTI-Luc 4 Lucia/Gaussia following manufacturer’s protocol. .. RNA in tissues and cells was extracted with Trizol (Invitrogen.



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    Image Search Results


    Selective activation of SBT777101 Jurkat reporter cells by citrullinated proteins. Representative dose–response curves showing fold-increase in luminescence ( y -axis) relative to baseline following incubation with increasing concentrations (log scale, x -axis) of citrullinated (red) or noncitrullinated (black) protein targets. Data points represent the mean ± SD of 3 replicates.

    Journal: Toxicological Sciences

    Article Title: Nonclinical safety assessment of SBT777101, a CAR-engineered regulatory T cell therapy for autoimmune diseases

    doi: 10.1093/toxsci/kfag125

    Figure Lengend Snippet: Selective activation of SBT777101 Jurkat reporter cells by citrullinated proteins. Representative dose–response curves showing fold-increase in luminescence ( y -axis) relative to baseline following incubation with increasing concentrations (log scale, x -axis) of citrullinated (red) or noncitrullinated (black) protein targets. Data points represent the mean ± SD of 3 replicates.

    Article Snippet: NFAT-luciferase reporter Jurkat cells (BPS Bioscience) were stably transduced with the SBT777101 CAR.

    Techniques: Activation Assay, Incubation

    Evaluation of the HEK-Blue IL-1R reporter platform for IL-1β detection. ( A ) Experimental protocol used to assess the dynamic range in response to IL-1β. ( B ) Statistical comparison among IL-1β standards ranging from 0.01 to 10,000 pg/mL as measured by the HEK-Blue IL-1R system. ( C ) Nonlinear four-parameter logistic (4PL) regression model generated from IL-1β standards. ( D ) Experimental workflow to obtain IL-1β-containing supernatants from BMMs in the presence or absence of LPS (10 ng/mL) and SEAP quantification using the HEK-Blue IL-1R assay. ( E ) Detection of SEAP activity over the course of a 5–15 min incubation with QUANTI-Blue reagent. N = 3, **** p < 0.0001, ns = not significant.

    Journal: Molecules

    Article Title: Cell Reporter-Based Analysis of Anti-Inflammasome Activities of Flavonoids with Distinct Structures

    doi: 10.3390/molecules31183176

    Figure Lengend Snippet: Evaluation of the HEK-Blue IL-1R reporter platform for IL-1β detection. ( A ) Experimental protocol used to assess the dynamic range in response to IL-1β. ( B ) Statistical comparison among IL-1β standards ranging from 0.01 to 10,000 pg/mL as measured by the HEK-Blue IL-1R system. ( C ) Nonlinear four-parameter logistic (4PL) regression model generated from IL-1β standards. ( D ) Experimental workflow to obtain IL-1β-containing supernatants from BMMs in the presence or absence of LPS (10 ng/mL) and SEAP quantification using the HEK-Blue IL-1R assay. ( E ) Detection of SEAP activity over the course of a 5–15 min incubation with QUANTI-Blue reagent. N = 3, **** p < 0.0001, ns = not significant.

    Article Snippet: HEK-Blue IL-1R reporter cells were obtained from InvivoGen (San Diego, CA, USA).

    Techniques: Comparison, Generated, Activity Assay, Incubation

    Application of the IL-1R HEK-Blue platform to compare the effects of flavonoids. ( A ) Experimental workflow for the detection of IL-1β in supernatants from BMMs treated with flavonoids and/or LPS using IL-1R HEK-Blue cells. ( B ) Comparison of SEAP activity, measured as OD at 640 nm, in Hek-Blue supernatants following exposure to media from BMMs cultured with or without LPS (10 ng/mL, 24 h). ( C ) Effects of flavonoid pretreatment (5–50 µM; NOB, QUER, or RUT) on SEAP induction by supernatants from LPS-treated BMMs. The −LPS and +LPS dashed lines indicate the mean OD values at 10 min from panel ( B ). ( D ) Comparison of OD values in Hek-Blue cell supernatants following exposure to supernatants from BMMs pretreated with flavonoids (10 µM). N = 12 for the −LPS and + LPS treatment groups and n = 4 for the flavonoid pretreatment groups. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Journal: Molecules

    Article Title: Cell Reporter-Based Analysis of Anti-Inflammasome Activities of Flavonoids with Distinct Structures

    doi: 10.3390/molecules31183176

    Figure Lengend Snippet: Application of the IL-1R HEK-Blue platform to compare the effects of flavonoids. ( A ) Experimental workflow for the detection of IL-1β in supernatants from BMMs treated with flavonoids and/or LPS using IL-1R HEK-Blue cells. ( B ) Comparison of SEAP activity, measured as OD at 640 nm, in Hek-Blue supernatants following exposure to media from BMMs cultured with or without LPS (10 ng/mL, 24 h). ( C ) Effects of flavonoid pretreatment (5–50 µM; NOB, QUER, or RUT) on SEAP induction by supernatants from LPS-treated BMMs. The −LPS and +LPS dashed lines indicate the mean OD values at 10 min from panel ( B ). ( D ) Comparison of OD values in Hek-Blue cell supernatants following exposure to supernatants from BMMs pretreated with flavonoids (10 µM). N = 12 for the −LPS and + LPS treatment groups and n = 4 for the flavonoid pretreatment groups. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Article Snippet: HEK-Blue IL-1R reporter cells were obtained from InvivoGen (San Diego, CA, USA).

    Techniques: Comparison, Activity Assay, Cell Culture